mouse dshb f55a10 pdx1 (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank
mouse dshb f55a10 pdx1
Mouse Dshb F55a10 Pdx1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-pdx1/anti-Homeobox+protein+Nkx-6%2E1/pmc11464881__41467_2024_53068_MOESM1_ESM-77-8-9
Average 95 stars, based on 85 article reviews
Mouse Dshb F55a10 Pdx1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-pdx1/anti-Homeobox+protein+Nkx-6%2E1/pmc11464881__41467_2024_53068_MOESM1_ESM-77-8-9
Average 95 stars, based on 85 article reviews
mouse dshb f55a10 pdx1 - by Bioz Stars,
2026-09
95/100 stars
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Expressing:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Staining:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse ChIP-sequencing:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Binding Assay:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Luciferase:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Activity Assay:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Transfection:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Plasmid Preparation:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Mutagenesis:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Transduction:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Sequencing:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Western Blot:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Ex Vivo:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Enzyme-linked Immunosorbent Assay:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Cell Function Assay:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Immunofluorescence:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Quantitation Assay:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Cell Culture:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Blocking Assay:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse RNA Sequencing Assay:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse TUNEL Assay:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse Activation Assay:Article Title: p43, a Truncated Form of Thyroid Hormone Receptor α, Regulates Maturation of Pancreatic β Cells Article Snippet: The following antibodies were used: guinea pig anti-insulin (1/250; ab7842 Abcam, Cambridge, UK), mouse anti-glucagon (1/100; ab10988 Abcam), rabbit anti-MafA (1/100; IHC-00352 Bethyl Laboratories, Montgomery, TX, USA), Article Title: Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling Article Snippet: Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), Article Title: Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors Article Snippet: The following primary antibodies were used: rabbit anti-Pdx1 (1:200), rabbit anti-MafA (1:200), rabbit anti-insulin (1:300), mouse anti-C-peptide (1:100), mouse anti-glucagon (1:200) (Abcam, Cambridge, United Kingdom), mouse anti-Neurogenin3 (1:800), Article Title: The Transcription Factor Myt3 Acts as a Pro-Survival Factor in β-cells Article Snippet: Sections were co-stained with rabbit anti-Myt3 (1/250) and guinea pig anti-Insulin (1/1000; Linco), guinea pig anti-Glucagon (1/1000; Linco), guinea pig anti-PP (1/100; Linco), goat anti-Somatostatin (1/1000; Santa Cruz) or Article Title: ALDH1B1 is a potential stem / progenitor marker for multiple pancreas progenitor pools Article Snippet: Primary antibodies used were: rabbit anti–ALDH1B1 (1:500) ( Stagos et al., 2010 ) rabbit anti-NGN3 (1:200; gift from H. Edlund), rat anti-E-CADHERIN (1:400; Zymed), rat anti-CYTOKERATIN 19 (anti-CK-19; 1:250; DSHB), mouse anti-INSULIN (1:1,000; Sigma), mouse anti-GLUCAGON (1:500; Sigma), mouse anti-BrdU (1:200 DAKO), Article Title: VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus Article Snippet: The following primary antibodies were used: guinea pig anti-insulin (1:300; Millipore), mouse anti-insulin (1:300; Sigma), guinea pig anti-insulin (1:200; Dako North America, Inc.), rabbit anti-glucagon (1:300), guinea pig anti-glucagon (1:300; Millipore), mouse anti-glucagon (1:500; Sigma), rabbit anti-Glut2 (1:500), rabbit anti-Sox9 (1:200; Chemicon), rat anti-somatostatin (1:200; Millipore), mouse |
Figure S2 (n = 3 independent donor samples). (G) qRT-PCR of pseudoislets, CRISPR-PDX1 (red), normalized to the CRISPR-Control (n = 3 independent donors). (H) Total Insulin content of CRISPR/Cas9 electroporated cells normalized to genomic DNA (gDNA) content (n = 4 independent donor samples). Data are presented as mean values ±SE. Two-tailed t tests were used to generate p values. See also 
